SKCCC Microarray Core Facility

 
array platforms and services

Agilent 4x 44K or 8x 60K Expression Array

Assays are for genome-scale RNA expression analysis. Assays are carried out with either duo-color labeling which allows two samples in comparison to be co-hybridized on an array or single-color labeling. Array service cost includes array purchase, RNA quality check, RNA amplification/labeling, array hybridization, image and data acquisition. Sample RNA isolation, consultation on experimental design and preliminary data analysis are not included but available upon request. Relevant array information can be found at Agilent's website: http://www.genomics.agilent.com/en/Gene-Expression-Microarrays/?pgid=AG-PG-6&navAction=pop

Sample requirement: 50500 ng total RNA. Minimum 4-pair samples for duo-color and 4 samples for single-color. Service price: See Price List

 

Agilent 4x 180K or 1M Array CGH

Assays are for genome-scale DNA copy number alteration analysis. Assays are carried out with duo-color labeling and co-hybridization of sample and reference DNA. Array service cost includes array purchase, genomic DNA quality check, DNA fragmentation, labeling, array hybridization, image and data acquisition. Core provides commercial reference genomic DNA if desired. Sample DNA isolation and preliminary data analysis are not included but available upon request. Relevant array information can be found at Agilent's website: http://www.genomics.agilent.com/en/CGH-CGH-SNP-Microarrays/?pgid=AG-PG-9&navAction=pop

Sample requirement: 1 - 3 ug genomic DNA. Minimum 4 samples for 4x 180K array and one sample for 1M array. Service price: See Price List

 

Agilent 8x 15K or 8x 60K MicroRNA Expression Array

Assays are for genome-scale microRNA expression analysis. Assays are carried out with single-color labeling. Samples can be submitted as total RNA or enriched small RNA isolated using specified methods (see Sample Preparation Protocols). Array service cost includes array purchase, RNA quality check, RNA labeling, array hybridization, image and data acquisition. Sample RNA isolation and preliminary data analysis are not included but available upon request. Relevant array information can be found at Agilent's website: http://www.genomics.agilent.com/en/miRNA-Microarrays/?pgid=AG-PG-5&navAction=pop

Sample requirement: 100 - 500 ng total RNA. Minimum 8 samples. Accept RNA isolated from paraffin-embedded samples. Service price: See Price List

 

Illumina HumanHT-12, MouseRef-8 or MouseWG-6 Expression BeadArray

Assays are for genome-scale RNA expression analysis. Assays are carried out using Illumina array kits and according to Illumina's protocols. Service cost includes array purchase, RNA quality check, amplification and labeling, array hybridization and data acquisition. Sample RNA isolation and preliminary data analysis are not included but available upon request. Relevant array information can be found at Illumina website: http://applications.illumina.com/applications/transcriptome-analysis/gene-expression-analysis/gene-expression-arrays.html

Sample requirement: 50 - 500 ng total RNA. Minimum 6, 8 or 12 samples depending on array type. Service price: See Price List

 

Illumina Infinium Methylation450 BeadArray

Assay is for genome-scale DNA methylation analysis. Assay is carried out using Illumina array kit and according to its protocols. Samples can be submitted as genomic DNA or bisulfite-converted DNA (see bisulfite conversion method in Sample Preparation Protocols). Service cost includes array purchase, DNA quality check, bisulfite conversion, DNA amplification, array hybridization and data acquisition. Relevant array information can be found at Illumina website: http://products.illumina.com/products/methylation_450_beadchip_kits.html

Sample requirement: 100 - 500 ng genomic DNA or 100 ng bisulfite-converted DNA. Minimum 12 samples. Accept DNA isolated from paraffin-embedded samples. Service price: See Price List

 

Illumina Omni Whole-Genome SNP BeadArrays

Assays are for genome-scale single-nucleotide polymorphism (SNP) and copy number variation (CNV) analysis. Assays are carried out using Illumina array kits and according to Illumina's protocols. Samples can be submitted as genomic DNA. Service cost includes array purchase, DNA quality check, DNA amplification, array hybridization and data acquisition. Relevant array information can be found at Illumina website: http://applications.illumina.com/applications/genotyping/human-genotyping-arrays.html?

Sample requirement: 200 ng genomic DNA. Minimum 4 - 24 samples deponding on array type. Accept DNA isolated from paraffin-embedded samples. Service price: See Price List

 

TaqMan RT-PCR Array Plates

Assays are for microarray target validation. Targets to be validated are chosen by array users. Minimum target number to be validated needs to be larger than 10. Core provides service on plate design, plate purchase, carrying out RT-PCR and data acquisition. 

Sample requirement: Depend on plate design. Consult with core manager. Service price: Consult with core manager.